Gene description for FLOT2 |
Gene name |
flotillin 2 |
Gene symbol |
FLOT2 |
Other names/aliases |
ECS-1 ECS1 ESA ESA1 M17S1 |
Species |
Homo sapiens |
Experiment description of studies that identified FLOT2 in Breast cancer cells |
1
|
Experiment ID | 210 |
Identified molecule | protein
|
Extracellular vesicle type | Microvesicles |
MISEV | EVs |
Identification method | Western blotting
|
PubMed ID |
21368175
|
Organism | Homo sapiens |
Experiment description | Cancer cell-derived microvesicles induce transformation by transferring tissue transglutaminase and fibronectin to recipient cells |
Authors | Antonyak MA, Li B, Boroughs LK, Johnson JL, Druso JE, Bryant KL, Holowka DA, Cerione RA |
Journal name |
Proc Natl Acad Sci U S A
|
Publication year | 2011 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 |
Isolation/purification methods | Differential centrifugation Filtration |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Immunofluorescent microscopy Scanning electron microscopy Mass spectrometry [4000 Q Trap] |
EV-TRACK |
-
|
|
|
2
|
Experiment ID | 575 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry [LTQ]
|
PubMed ID |
27894104
|
Organism | Homo sapiens |
Experiment description | Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. |
Authors | Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. |
Journal name |
Oncotarget
|
Publication year | 2016 |
Sample | Breast cancer cells |
Sample name | BT549 |
Isolation/purification methods | Differential centrifugation Polymer-based precipitation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [LTQ] |
EV-TRACK |
-
|
|
|
3
|
Experiment ID | 579 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry [LTQ]
|
PubMed ID |
27894104
|
Organism | Homo sapiens |
Experiment description | Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. |
Authors | Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. |
Journal name |
Oncotarget
|
Publication year | 2016 |
Sample | Breast cancer cells |
Sample name | MDA-MB-468 |
Isolation/purification methods | Differential centrifugation Polymer-based precipitation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [LTQ] |
EV-TRACK |
-
|
|
|
4
|
Experiment ID | 1957 |
Identified molecule | protein
|
Extracellular vesicle type | Microvesicles |
MISEV | Large EVs |
Identification method | Western blotting
|
PubMed ID |
36475545
|
Organism | Homo sapiens |
Experiment description | Weakly migratory metastatic breast cancer cells activate fibroblasts via microvesicle-Tg2 to facilitate dissemination and metastasis |
Authors | Schwager SC, Young KM, Hapach LA, Carlson CM, Mosier JA, McArdle TJ, Wang W, Schunk C, Jayathilake AL, Bates ME, Bordeleau F, Antonyak MA, Cerione RA, Reinhart-King CA. |
Journal name |
Elife
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 - weakly migratory (MDA- ) |
Isolation/purification methods | Centrifugation Filtration |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Mass spectometry |
EV-TRACK |
-
|
|
|
5
|
Experiment ID | 1958 |
Identified molecule | protein
|
Extracellular vesicle type | Microvesicles |
MISEV | Large EVs |
Identification method | Western blotting
|
PubMed ID |
36475545
|
Organism | Homo sapiens |
Experiment description | Weakly migratory metastatic breast cancer cells activate fibroblasts via microvesicle-Tg2 to facilitate dissemination and metastasis |
Authors | Schwager SC, Young KM, Hapach LA, Carlson CM, Mosier JA, McArdle TJ, Wang W, Schunk C, Jayathilake AL, Bates ME, Bordeleau F, Antonyak MA, Cerione RA, Reinhart-King CA. |
Journal name |
Elife
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 - highly migratory (MDA+) |
Isolation/purification methods | Centrifugation Filtration |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Mass spectometry |
EV-TRACK |
-
|
|
|
6
|
Experiment ID | 1959 |
Identified molecule | protein
|
Extracellular vesicle type | Microvesicles |
MISEV | Large EVs |
Identification method | Western blotting
|
PubMed ID |
36475545
|
Organism | Homo sapiens |
Experiment description | Weakly migratory metastatic breast cancer cells activate fibroblasts via microvesicle-Tg2 to facilitate dissemination and metastasis |
Authors | Schwager SC, Young KM, Hapach LA, Carlson CM, Mosier JA, McArdle TJ, Wang W, Schunk C, Jayathilake AL, Bates ME, Bordeleau F, Antonyak MA, Cerione RA, Reinhart-King CA. |
Journal name |
Elife
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 - weakly migratory (MDA- ) |
Isolation/purification methods | Centrifugation Filtration |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Mass spectometry |
EV-TRACK |
-
|
|
|
7
|
Experiment ID | 1960 |
Identified molecule | protein
|
Extracellular vesicle type | Microvesicles |
MISEV | Large EVs |
Identification method | Western blotting
|
PubMed ID |
36475545
|
Organism | Homo sapiens |
Experiment description | Weakly migratory metastatic breast cancer cells activate fibroblasts via microvesicle-Tg2 to facilitate dissemination and metastasis |
Authors | Schwager SC, Young KM, Hapach LA, Carlson CM, Mosier JA, McArdle TJ, Wang W, Schunk C, Jayathilake AL, Bates ME, Bordeleau F, Antonyak MA, Cerione RA, Reinhart-King CA. |
Journal name |
Elife
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 - weakly migratory (MDA- ) |
Isolation/purification methods | Centrifugation Filtration |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Mass spectometry |
EV-TRACK |
-
|
|
|
8
|
Experiment ID | 1664 |
Identified molecule | Protein
|
Extracellular vesicle type | Exosomes |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
34108659
|
Organism | Homo sapiens |
Experiment description | Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors | Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
Journal name |
Nat Cell Biol
|
Publication year | 2021 |
Sample | Breast cancer cells |
Sample name | MCF7 |
Isolation/purification methods | Differential centrifugation Filtration Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein mRNA |
Methods used in the study | RT-qPCR Western blotting Mass spectrometry Flow cytometry |
EV-TRACK |
-
|
|
|
9
|
Experiment ID | 1666 |
Identified molecule | Protein
|
Extracellular vesicle type | Exosomes |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
34108659
|
Organism | Homo sapiens |
Experiment description | Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors | Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
Journal name |
Nat Cell Biol
|
Publication year | 2021 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 |
Isolation/purification methods | Differential centrifugation Filtration Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein mRNA |
Methods used in the study | RT-qPCR Western blotting Mass spectrometry Flow cytometry |
EV-TRACK |
-
|
|
|
10
|
Experiment ID | 1678 |
Identified molecule | Protein
|
Extracellular vesicle type | Exosomes |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
34108659
|
Organism | Homo sapiens |
Experiment description | Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors | Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
Journal name |
Nat Cell Biol
|
Publication year | 2021 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods | Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Mass spectrometry |
EV-TRACK |
-
|
|
|
11
|
Experiment ID | 1678 |
Identified molecule | Protein
|
Extracellular vesicle type | Exosomes |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
34108659
|
Organism | Homo sapiens |
Experiment description | Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors | Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
Journal name |
Nat Cell Biol
|
Publication year | 2021 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods | Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Mass spectrometry |
EV-TRACK |
-
|
|
|
12
|
Experiment ID | 1679 |
Identified molecule | Protein
|
Extracellular vesicle type | Exosomes |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
34108659
|
Organism | Homo sapiens |
Experiment description | Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors | Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
Journal name |
Nat Cell Biol
|
Publication year | 2021 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods | Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
-
|
|
|